Research Paper Volume 11, Issue 3 pp 921—932

MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma

Figure 2. EGFR is a direct target of miR-141. (A) Prediction of miR-141 binding sites in the EGFR 3’-UTR. (B) Interaction between miR-141 and the EGFR 3’-UTR in FaDu and Cal-27 cells tested in luciferase assays. AS, antisense. (C) Western blots showing expression of EGFR in INOK, FaDu and Cal-27 cells. (D) Western blots showing expression of miR-141 and EGFR in INOK, FaDu and Cal-27 cells. (E, F) Western blot and real-time PCR showing co-expression of EGFR in FaDu cells with miR-141 mimic or inhibitor. (G, H) Western blot and real-time PCR showing co-expression of EGFR in Cal-27 with miR-141 mimic or inhibitor. Bars depict the mean ± SD. ** P< 0.05 vs. INOK cells (C, D) or control (E, F).