Research Paper Volume 11, Issue 4 pp 1110—1128

The ubiquitin ligase KBTBD8 regulates PKM1 levels via Erk1/2 and Aurora A to ensure oocyte quality

Figure 3. KBTBD8 is enriched in the ovary, oocytes, and meiotic spindles. A. Oocyte expression of KBTBD protein family mRNAs. Strong expression of KBTBD6, 7, and 8 was observed. B. KBTBD8 protein expression by western blot in mouse ovaries and testis. Higher KBTBD8 levels were seen in ovaries. β-actin was used as loading control. C. KBTBD8 is expressed in oocytes but undetectable in granular cells (Gc). β-actin was used as loading control for western blot. D. Chronological expression of KBTBD8 in the mouse ovary. β-actin was used as loading control for western blot. PND: post-natal day. E. KBTBD8 expression during oocyte meiosis. Tubulin was used as loading control for western blot. F. KBTBD8 immunofluorescence during meiotic stages. KBTBD8 is abundant within the nucleus at GV, enriched near chromosomes at GVBD, and concentrated at the spindle poles at MI and MII. MTs: microtubules. Scale bar, 20 µm.