Figure 5. KBTBD8 is essential for normal fertilization. A. Immunofluorescence assessment of fertilization. Most control oocytes showed the normal 2 pronuclei (PN), while most fertilized oocytes in the KBTBD8-depleted group had abnormal pronuclei numbers (0-PN, 1-PN, or 3-PN). MTs: microtubules. Scale bar, 20 µm. B. Quantification of fertilization rate (fertilized oocytes/total oocytes) and 2-PN rate (2-PN oocytes/fertilized oocytes). *p ˂ 0.05.