Research Paper Volume 11, Issue 3 pp 1019—1029

A comprehensive evaluation of 181 reported CHST6 variants in patients with macular corneal dystrophy

Figure 3. Multiple sequence alignment result of CHST6 protein. Protein sequences for CHST6 retrieved from NCBI for human, chimpanzee, mouse, chicken, frog and zebrafish showed amino acid conservation among different vertebrate species (for mouse, the sequence of CHST5 was used here). The sulfotransferase domain (residue 42-356) was labeled in cyan, and the two PAPS binding sites (residue 49-55 and 202-210) were labeled in carmine. Arrowheads indicated amino acid changes caused by reported human mutations. Strongly conserved positions were labeled with red or orange arrowheads, while weakly conserved ones were labeled with blue or green arrowheads (annotated by Clustal Omega).