Figure 5. Effect of EO on ROS assay and SOD2 gene expression. Addition of EO lowered ROS levels in AMD cybrids at 24 h (A), 48 h (B), and 72 h (C) time points. (D) shows increased expression of the antioxidant gene, SOD2, as a result of treatment with EO. ** and * indicate p<0.01 and p<0.05 respectively. Data are presented as mean ± SEM and normalized to untreated AMD cybrids which were assigned a value of 1.