Research Paper Volume 11, Issue 4 pp 1226—1239

Methane alleviates sepsis-induced injury by inhibiting pyroptosis and apoptosis: in vivo and in vitro experiments

Figure 2. Methane-rich saline alleviated the histopathology damage and organ dysfunction induced by sepsis. The lung and intestine tissues were collected 12 and 24 h after the cecal ligation and puncture (CLP) operation. (A) Representative hematoxylin and eosin (H&E) staining of lung and intestine sections (Scale bars: 50μm). (B) The lung wet/dry weight ratio was calculated as an assessment of lung injury. (C) Lung injury scores. (D) The villus height was measured as an assessment of intestine injury. (E) Intestine injury scores. (n = 12. Data are shown as the mean ± SD. *P < 0.05; **p < 0.01; ***P < 0.001).