Figure 4. Eﬀects of methane on antioxidants and oxidative products. The lung and intestine tissues were harvested 12 and 24 h after cecal ligation and puncture (CLP) (n = 12). The (A-B) Myeloperoxidase (MPO), (C-D) malonaldehyde (MDA), (E-F) glutathione (GSH) and (G-H) superoxide dismutase (SOD) in lung and intestine were detected 12 and 24h after CLP. (I–J) Representative dihydroethidium (DHE) fluorescence staining in RAW 264.7 cells and the calculated fluorescence intensity (Scale bars: 25 μm and 50 μm) (n = 3). (Data are shown as the mean ±SD. *P < 0.05; **p < 0.01; ***P < 0.001; #P < 0.05 versus normal medium (NM) group; $p < 0.05 versus methane-rich medium (MRM) group; &P < 0.05 versus. normal medium+ lipopolysaccharide+ adenosine-triphosphate (NM+L+A) group).