Figure 6. Methane-rich saline downregulated the NLRP3/Caspase-1/IL-18, IL-1β signaling pathway. The lung tissues were harvested 12 and 24 h after cecal ligation and puncture (CLP). (A) Representative immunoblots of p-p65, p65, pro-Caspase-1, pro-IL-1β and NLRP3/Caspase-1/ IL-1βfrom lung tissues 12 and 24h after MRS treatment. Relative densities of (B) p-P65. (C) P65. (D) NLRP3. (E) caspase-1. (F) pro-caspase-1. (G) IL-1β. (H) pro-IL-1β were calculated. (I) Representative immumohistochemical staining of P65, caspse-1 and IL-1β in the lung and intestine tissues. (J-L) The percentage of P65, caspse-1 and IL-1β positive cells were calculated. (Scale bars: 50μm. Data are shown as the mean ±SD. *P < 0.05; **p < 0.01; ***P < 0.001).