Research Paper Volume 11, Issue 5 pp 1342—1355

MicroRNA-127 inhibits cell proliferation via targeting Kif3b in pancreatic β cells

Figure 1. miR-127 was dominantly expressed in mouse islets. (A) QRT-PCR analysis of relative miR-127 expression in various isolated tissues from 8-week-old C57BL/6 mice (n=4). The level of miR-127 in mouse islets were measured in 5-week (B) and 20-week (C) old HFD mice and age-matched NFD mice (n=4) by qRT-PCR. NFD, normal fat diet; HFD, high fat diet. (D) MIN6 cells were cultured with the indicated concentrations of glucose (5.5, 11.1, 25, or 33.3mM) for 24 h. Subsequently, miR-127 expression was measured by qRT-PCR. U6 was used as the internal control. The values are presented as the means ± SD. *p<0.05.