Research Paper Volume 11, Issue 5 pp 1342—1355

MicroRNA-127 inhibits cell proliferation via targeting Kif3b in pancreatic β cells

Figure 2. Overexpression of miR-127 attenuated cell proliferation and insulin secretion. MIN6 cells and primary islet cells were transfected with miR-127 mimics, miR-127 inhibitor or matched control. Forty-eight hours after transfection with miR-127 mimics, MIN6 cell viability was assayed by the CCK-8 assay (A) and EDU assay (B). CCK-8 assay (C) and EDU assay (D) were used to determine cell viability in MIN6 cells after transfection with miR-127 inhibitor. Primary islet cells proliferation was measured after transfection with miR-127 mimics (E) and miR-127 inhibitor (F) using CCK-8 assay. (G) Apoptosis was evaluated by flow cytometry with Annexin V-FITC and PI staining, and the quantitative analysis of apoptotic cell frequency was shown. (H) MIN6 cells were harvested and cycle distribution monitored with flow cytometry, and a quantitative analysis of the cell cycle distribution was shown. Relative level of insulin secreted in the supernatant (I, J) and cellular insulin content (K, L) were measured by ELISA and normalized by total protein content. The level of insulin in miR-127 NC group was defined as 1 and the results were shown as a relative fold of increase. The values are presented as the means ± SD. *p<0.05.