Research Paper Volume 11, Issue 5 pp 1342—1355

MicroRNA-127 inhibits cell proliferation via targeting Kif3b in pancreatic β cells

Figure 3. miR-127 suppressed cell proliferation by targeting Kif3b. Effects of overexpressed miR-127 on Kif3b mRNA (A) and protein expression (B) were estimated by qRT-PCR and Western blot respectively. (C) Schematic representation shows the predicted target sites of miR-127 at the 3′-UTR of Kif3b. A mutation in the seed matches is refereed as Kif3b-MUT. (D) Luciferase assay of the reporter plasmids harboring the intact or mutant Kif3b-3′UTR in MIN6 cells. EDU assay (E) and CCK-8 assay (F) were used to evaluate MIN6 cell proliferation. (G) CCK-8 assay was applied to evaluate primary islet cells viability. Insulin secreted in the supernatant (H) and cellular insulin content (I) were measured by ELISA and normalized to total protein content. The level of insulin in Kif3b NC group was defined as 1 and the results were shown as a relative fold of increase. The values are presented as the means ± SD. *p<0.05.