Figure 4. Characteristics of EVs collected from the medium of MIN6 cells. (A) Nanoparticle tracking analysis displayed the size distribution and concentration of particles isolated from MIN6 cells. (B) EVs were observed by transmission electron microscopy. Scale bar, 200 nm. (C) Western blot analysis of exosomal markers in isolated particles and whole MIN6 cell lysates. (D) Uptake of EVs in MS1 cells by confocal microscopy. Blue: DAPI staining; green: PKH67-labeled EVs. (E) The level of miR-127 in MS1 cells was measured after co-culturing with EVs for 24 h by qRT-PCR. The values are presented as the means ± SD. *p<0.05.