Figure 4. SRSF3 downregulation leads to senescence-associated phenotypes in both human and mouse cells. (A) SA-β-Gal staining before and after knockdown with two shRNAs (sh1 and sh2). (B) CCK-8 assay for cells with or without knockdown of SRSF3 in human and mouse cells. (C) Cell cycle analysis before and after knockdown of SRSF3 in 293T and MEF cells. (D) qRT-PCR revealed that knockdown of SRSF3 led to decreased expression of cell proliferation markers (MKI67, CDK1) and increased expression of senescence marker (CDKN1A or CDKN1B) in both human (293T and HUVEC) and mouse (MEFs and NIH3T3) cells. *, ** and *** mean P value less than 0.05, 0.01 and 0.001 (t-test), respectively.