Research Paper Volume 11, Issue 5 pp 1456—1470

Long intergenic non-coding LINC00657 regulates tumorigenesis of glioblastoma by acting as a molecular sponge of miR-190a-3p

Figure 9. In vivo study on inducing cell apoptosis effect of LINC00657. (A) The pcDNA3.1-LINC00657 transfected LN-18 and U-118MG cells were subcutaneously injected to nude mice, and the tumor volume were measured weekly. The tumor volume was equal to length × width2 × 0.5. (B) Tumors were isolated from xenografts in 4 weeks. (C) Tumor weights in each group were quantified. (D) TUNEL staining of tumor tissues in each group. (E) TUNEL positive cell rate in each group was quantified. (F) Relative level of LINC00657 in each group was detected with qPCR. (G) Relative expressions of PTEN and active-caspase 3 in tumors were detected with western blot assay. (H, I) Relative expressions of PTEN and active-caspase 3 in tumors were quantified with Image-Pro Plus. *P<0.05, **P<0.01.