Figure 1. Preparation of primary mouse gastric cancer cells. (A) Schematic: H. felis was given to INSp-GAS (expression of gastrin under insulin promoter) transgenic mice, after which gastric tumor tissue was confirmed by histology after dissection out the formed tumor. (B) Representative histology of normal stomach and GC. (C) A pooled primary gastric cancer (PGC) cell fraction was prepared from 10 mice that successfully generated tumor, after a negative selection for CD45+ inflammatory cells, and CD31+ tumor endothelial cells. Scale bars are 100µm.