Figure 3. The effect of age on circulating levels of RAGE ligands: AGEs, S100A8/A9 and HMGB1. (A) Bars represent serum levels of indicated RAGE ligands in three age groups of a healthy population, young (≤45 yrs; n=36), middle age (46-64 yrs; n=65-71) and elderly-old (≥65 yrs; n=57-61). Data are in the original units. Values are expressed as median and interquartile range. Kruskal-Wallis with Dunn’s multiple comparison test. (B) Scatter plots showing correlation between age and HMGB1, AGEs or S100A8/A9. Variables showed a skew-ness distribution and were log-transformed. r=Pearson’s coefficient. P < 0.05 was considered statistically significant; n=159-169.