Research Paper Volume 11, Issue 6 pp 1759—1777

Tubastatin A inhibits HDAC and Sirtuin activity rather than being a HDAC6-specific inhibitor in mouse oocytes

Figure 4. Mechanistic target of maturation promoting factor (MPF) signaling is dysregulated in TubA-treated oocytes. (A) Cyclin-dependent kinase (Cdk1, 2, 4, 6, Cdc25b, and Ccnb1) mRNA expression profiles using IGV. (B) Confocal immunofluorescence analysis of CDK1 (green) and Cyclin B (red) in oocytes with/without TubA exposure during in vitro maturation at 0 h, 9 h, and 12 h. The experiment was repeated three times. DNA was stained with DAPI (blue). Right indicates quantification of each staining density. (C, D) mRNA expression profiles of cytokinesis- and MI arrest-related factors in mouse oocyte profiles in matched pairs of TubA (10 or 20 μM)-treated (TubA/GV pairs) versus untreated control groups (MII/GV pairs). (E) Confirmation of DEGs obtained from RNA-seq. Bar graph showing relative gene expression obtained from qRT-PCR analysis of selected genes. *, **, ***, and **** represent genes displaying statistically significant changes in expressions.