Figure 6. ET-1 was overexpressed in ETBR knockout GENs and was regulated by NF-kapapB pathway. GENs was cultured in HG medium, and the supernatant and GENs were collected at 6 h, 12 h, 16 h, 20 h, 24 h after cultivation. (A) Under the HG condition, ET-1 expression (in the supernatant)in WT and ETBR knockout GENs groups was detected at 6 h, 12 h, 16 h, 20 h, 24 hGENGEN . **p<0.01, compared with WT. There was significant difference in ET-1 production rate between ETBR knockout and WT GENs since 16 h, and the difference increased with time. ET-1 production rate (n)= ET-1(n)/ET-1(n-4). N represented the time point of sample collection. ET-1 represented the production of ET-1 in mesangial cells. *p<0.05 compared with WT. **p<0.01, compared with WT. (B) mRNA expressions of EDN1 in WT GENs and ETBR-/- GENs groups were detected at 6 h, 12 h, 16 h, 20 h, 24 h. **p<0.01, compared with 6h. mRNA expression of ET-1 was increased in ETBR-/- GENs within 24 h. **p<0.01, compared with 6h. (C) Protein levels of p-p65 in WT GENs and ETBR-/- GENs groups were measured at 6 h, 12 h, 16 h, 20 h, 24 h. Bars depict the mean ± SD. N=3.