Figure 3. SIRT1 is targeted by miR-124 in HCC. (A) Complementary sequence of SIRT1 3’ UTR paired with miR-124. (B) Expression of miR-124 in CD133+ and CD133- HepG2 and Huh7 cells was detected by qRT-PCR. (C) Effect of miR-124 mimics and inhibitors on changing the luciferase activities of pMIR plasmid contained SIRT1 3’ UTR. *P<0.05 vs. NCO group. (D) CD133+ and CD133- HepG2 and Huh7 cells were transfected with miR-124 (50 pmol/mL) or anti-miR-124 (50 pmol/mL) for 24h. Western blot analysis was then performed to detect the expression of SIRT1 in these cells. *P<0.05 vs. NCO group.