Research Paper Volume 11, Issue 6 pp 1821—1831

Reactivation of microRNA-506 inhibits gastric carcinoma cell metastasis through ZEB2

Figure 1. MiR-506 and ZEB2 expression in gastric carcinoma. (A) We performed miRNA microarray analysis to compare the miRNA expression profiles between gastric carcinoma tissues (GC) and paired non-cancerous gastric tissue (NGT). We set the cut-off level as fold change > 2 and P value < 0.05. The original intensities data were normalized using the Quantile method. The microarray analysis identified some unique differentially expressed miRNAs. (B) RT-qPCR for miR-506 in GC versus NGT. (C) Bioinformatics analysis showed that miR-506 targets ZEB2, which is a well-known factor that regulates cell invasiveness and migration, (D) Western blot for ZEB2 in GC versus NGT. (E) Correlation between miR-506 and ZEB2 in 26 gastric carcinoma specimens (ɤ= -0.77, p<0.0001). (F) The all 26 patients included in this study were followed up for 5 years for the overall survival. The median value of miR-506 in the 26 patients was used as a cutoff point to separate the total samples into miR-506-high group (n=13) and miR-506-low group (n=13). The Kaplan-Meier curves for the overall 5-year survival of these patients were shown. *p<0.05. **p<0.01. N=26.