Research Paper Volume 11, Issue 6 pp 1821—1831

Reactivation of microRNA-506 inhibits gastric carcinoma cell metastasis through ZEB2

Figure 2. MiR-506 targets 3’-UTR of ZEB2 mRNA to inhibit its expression. (A) We either overexpressed miR-506, or inhibited miR-506 in AGS cells by transfection of the cells with a miR-506-expressing plasmid (miR-506), or with a plasmid carrying an antisense for miR-506 (as-miR-506). The AGS cells were also transfected with a plasmid carrying a null sequence as a control (null). RT-qPCR for miR-506 was done in the transfected cells. (B) Western blot for ZEB2 in transfected AGS cells. (C–D) MiR-506-modified AGS cells were then transfected with wildtype (C) or mutate (D) ZEB2-3’-UTR luciferase-reporter plasmid, after which the luciferase activities were quantified. *p<0.05. NS: non-significant. N=5.