Research Paper Volume 11, Issue 6 pp 1850—1873

Longitudinal assessment of health-span and pre-death morbidity in wild type Drosophila

Figure 7. Dietary interventions can change late-life quality substantially. (A, B) Box plots depict ill-span duration for all 6 male (A) and all 6 female (B) populations raised on different diets. *** indicates statistical significance with p < 0.001, Kruskal Wallis ANOVA with Dunn’s posthoc testing. (C, D) Average disability scores of illderly flies plotted over the last 10 days of life for all 6 male (C) and all 6 female (D) populations. ** indicates statistical significance (p < 0.01, one way repeated measures ANOVA). (E, F) Duration of health-span (light gray bars), mild/moderate ill-span (dark gray bars), and severe/complete ill-span (black bars), as a percentage of lifespan are plotted for each diet and separately for males (E) and females (F). * indicates statistically significant difference (Kruskal Wallis ANOVA with Dunn’s posthoc testing (G, H). The relative percentages of animals without impairments (light gray bars), with mild impairments (medium gray bars) and with severe impairments (dark gray bars) during the last day of life plotted separately for each diet and males (G) and females (H). * indicates statistically significant differences (p < 0.05, Chi-square test). (I) The cumulative startle response score through the last ten days of life (late-life functional signature) plotted for each diet (color coded) and separately for females (front row) and males (back row).