Research Paper Volume 11, Issue 7 pp 1965—1976

SIRT6 participates in the quality control of aged oocytes via modulating telomere function

Figure 3. SIRT6 knockdown in oocytes results in the developmental delay of early embryos. (A) Schematic presentation of the SIRT6-knockdown experiments. (B) Efficiency of SIRT6 knockdown (SIRT6-KD) after siRNA injection was verified by immunoblotting. Tubulin served as a loading control. Band intensity was calculated using Image J software. (C) Representative contrast images of blastocyst embryos derived from control and SIRT6-KD oocytes. Red arrows indicate the developmental delay. (D) The percentage of zygotes that successfully progressed to the blastocyst stage during in vitro culture (n= 65 for control; n=55 for SIRT6-KD). Data are expressed as mean percentage ± SD of three independent experiments. Statistical analyses were performed with Student’s t-test. **P <0.01.