Figure 7. DMAMCL treatment suppresses NF-κB activities in multiple tissues and organs in old mice. The total protein was isolated from the heart, spleen, kidney, and fat tissues. The p-p65 and p65 protein expression levels in heart (A and B), and in spleen (C), the expression levels of IκBα, HO-1 and Nrf2 in kidney (D), and p-p65 and p65 protein expression levels in fat (E and F), were examined by Western blot (n=3-8). (G) TNF-α mRNA levels in fat tissue were detected by qRT-PCR. Data are represented as the mean ± SEM. *P < 0.05 compared with the control group (t-test two tailed).