Figure 3. TAZ is the target of miR-125b in glioma. (A) TargetScan, miRanda and PicTar databases were used to predict the binding site of miR-125b in TAZ 3′ UTR. (B) Expression level of miR-125b in U251, U251/R, U87 and U87/R cells. (C) U251, U251/R, U87 and U87/R cells were co-transfected with miR-125b mimics or inhibitors and wild-type (wt) or mutant (mt) TAZ 3′ UTR. Luciferase activities were measured by using Dual-Luciferase Reporter Assay System. *P<0.05 vs. NCO group. (D) Western blot analysis of TAZ expression in U251, U251/R, U87 and U87/R cells transfected with miR-125b mimics or inhibitors.