Research Paper Volume 11, Issue 9 pp 2670—2680

Downregulation of miR-125b promotes resistance of glioma cells to TRAIL through overexpression of Tafazzin which is a mitochondrial protein

Figure 6. MiR-125b mimics targets TAZ to attenuate the TRAIL resistance of glioma in vivo. (A) Tumor formation assays were performed with 7 mice per group. The tumor growth was monitored every four days. (B) Expression of miR-125b in the purified tumor tissues was detected by qRT-PCR analysis. *P<0.05 vs. U87/R group. #P<0.05 vs. U87/R + TRAIL group. (C) Expression of TAZ in the purified tumor tissues was detected by qRT-PCR and western blot analysis. *P<0.05 vs. U87/R group. #P<0.05 vs. U87/R + TRAIL group. (D) Protein level of cytochrome c in mitochondria or cytoplasm faction of purified tumor tissues.