Research Paper Volume 11, Issue 9 pp 2681—2698

Magnesium transporter protein solute carrier family 41 member 1 suppresses human pancreatic ductal adenocarcinoma through magnesium-dependent Akt/mTOR inhibition and bax-associated mitochondrial apoptosis

Figure 2. Overexpression of SLC41A1 suppresses tumour growth in an orthotopic mouse model of PDAC. (A) SLC41A1 protein was downregulated in human PDAC tissue. (B) SLC41A1 protein expression was suppressed in six PDAC tissue samples compared with paired non-adjacent pancreatic tissue. (C) SLC41A1 mRNA expression was suppressed in six PDAC tissues compared with paired non-adjacent pancreatic tissue. (D) Expression of SLC41A1 was downregulated in the human PDAC cell lines KP3, Panc-1, and BxPC3 compared with the normal pancreatic ductal epithelial cell line hTERT-HPNE. (E) Successful overexpression of SLC41A1 in KP3 cells that were used to create the orthotopic model (n = 5). (F) Overexpression of SLC41A1 suppressed the growth rate of orthotopic tumours in mice. (G) Tumour size was reduced by SLC41A1 overexpression. **p < 0.01; ***p < 0.001.