Research Paper Volume 11, Issue 9 pp 2699—2723

Laminin α4 overexpression in the anterior lens capsule may contribute to the senescence of human lens epithelial cells in age-related cataract

Figure 1. Senescence associated markers increase with the grades of age-related cataract (ARC) in human anterior lens capsules (ALCs). Human ALCs graded before surgery for cataract severity using the Emery-Little Classification System of nuclear opacity grade were obtained from the anterior surface of cataractous lenses during surgery. (A) Photographs for representing immunoblot analysis of p53 and corresponding Coomassie brilliant blue (CBB) staining in cataractous ALCs of different grades and ages. (B) Relative expression levels of p53 immunoblots in cataractous ALCs. Quantification of immunoblots was processed using Image J. The figure depicts Pearson correlation between age and p53 protein expression (n = 144). (C) The correlation between patient age and ARC grades (Data were analyzed via One-way ANOVA). (D) The correlation between relative p53 expression and ARC grades (Data are analyzed via Wilcoxon Rank Sum Test). Data were shown as mean ± SD. *, p<0.05; **, p<0.01; ***, p<0.001 versus grade II group. #, p<0.05 versus grade III group.