Research Paper Volume 11, Issue 9 pp 2699—2723

Laminin α4 overexpression in the anterior lens capsule may contribute to the senescence of human lens epithelial cells in age-related cataract

Figure 2. Excess laminins (LMs) in the senescent anterior lens capsules (ALCs) of age-related cataract (ARC). Representative photographs of hematoxylin and eosin (HE) staining of cataractous ALCs (A) and immunohistochemistry (IHC) of LMs in cataractous ALCs (B) (Scale bars: 50 μm). (C) Total LM in cataractous ALCs with different grades as detected by ELISA (Data were shown as mean ± SD and were analyzed by one-way ANOVA). ***, p<0.001 versus grade II group. The figure depicts Pearson correlation between total LM expression and relative p53 expression (n = 128) (D). (E) Immunoblot analysis of LM subunits in mixed cataractous ALCs (n = 10). (F) LM trimers in mixed cataractous ALCs as detected by LMα4. Immunoprecipitation-immunoblotting (IP-IB) using antibodies against LMα4 to precipitate LM trimers and antibodies against LMγ1, LMβ2 and LMβ1 for IB (n = 10).