Figure 3. Premature senescence model of human lens epithelial cells (HLE B-3) induced by H2O2. Cells of the control group were cultured in medium only, whereas cells of senescent group were cultured in medium with H2O2 for 96 h. (A) Viabilities (left) of HLE B-3 cells treated with different concentrations of H2O2 (0–600 μM) for 96 h, as measured via an MTT assay. Morphologic changes (right) of HLE B-3 cells following a 96 h exposure to 400 μM H2O2. (B) Percentage of SA-β-gal-positive cells in HLE B-3 cells treated with different concentrations of H2O2 (0–600 μM) (left). SA-β-gal activity as measured by cell staining (right). (C) Immunoblot analysis of GLB1, p21 and P53 in HLE B-3 cells. (D) Immunofluorescence analysis of p21 (green) in HLE B-3 cell nuclei. (Scale bars: 100 μm). Data were shown as mean ± SD and are representative of 3 independent experiments.