Research Paper Volume 11, Issue 9 pp 2699—2723

Laminin α4 overexpression in the anterior lens capsule may contribute to the senescence of human lens epithelial cells in age-related cataract

Figure 4. Elevated laminins (LMs) in senescent HLE B-3 cells and cell basement membranes (BMs) induced by H2O2. Cells (or cell BMs) of the control group were cultured in medium only, while cells (or cell BMs) of senescent group were cultured in medium with H2O2 (400 μM) for 96 h. (A) Total LM in HLE B-3 cells as detected by ELISA (data were analyzed by paired t-test). (B) Immunoblot analysis of 11 LM subunits, collagen 1α1, ATP1A1, MMP-9, and TGF-β1 in HLE B-3 cells. (C) Total LM in HLE B-3 cell BMs as detected by ELISA (data were analyzed by paired t-test). (D) SDS-PAGE analysis followed by CBB staining of HLE B-3 cell BMs. (E) Immunoblot analysis of LM subunits and MMP-9 in HLE B-3 cell BMs. (F) Immunofluorescence analysis of LMα4, LMβ3, and MMP-9 in HLE B-3 cell BMs (Scale bars: 50 μm). (G) TGF-β1 in HLE B-3 cell BMs, as detected by ELISA (data were analyzed using One-way ANOVA). Data are shown as mean ± SD. *, p<0.05; **, p<0.01 versus control group.