Research Paper Volume 11, Issue 9 pp 2735—2748

RON and RONΔ160 promote gastric cancer cell proliferation, migration, and adaption to hypoxia via interaction with β-catenin

Figure 2. Effects of RON/RONΔ160 on the activation of β-catenin signaling in human gastric cancer cell lines. (A) Relative mRNA levels of RON and β-catenin signaling-related genes detected in human gastric cancer cell lines using qPCR. (B) Western blots showing RON, β-catenin, TCF4, c-Myc, Cyclin D1, and survivin in three gastric cancer cell lines. (C) Transfection efficiency of RON and RONΔ160 in MGC-803 cells. (D) Expression of β-catenin signaling-related genes after transfection of RON or RONΔ160 into MGC-803 cells. (E) Interaction of RON/RONΔ160 and β-catenin in MGC-803 cells determined using co‑immunoprecipitation assays. ** p<0.01 vs Blank group.