Research Paper Volume 11, Issue 9 pp 2735—2748

RON and RONΔ160 promote gastric cancer cell proliferation, migration, and adaption to hypoxia via interaction with β-catenin

Figure 4. Effect of hypoxia on HIF-1α binding to the RON/RONΔ160-β-catenin complex. (A) Western blots of p-RON, RON, RONΔ160, β-catenin, and HIF-1α in KATOIII cells under normoxic and hypoxic conditions. (B) Immunostaining showing nuclear localization of RON, RONΔ160, β-catenin, and HIF‑1α in KATOIII cells under normoxic and hypoxic conditions. Scale bar = 50 μm. (C) Interaction between HIF-1α and the RON/RONΔ160-β-catenin complex in KATOIII cells under normoxic and hypoxic conditions. (D) Western blots showing suppression of RON and β-catenin in KATOIII cells transfected with siRNAs targeting RON or β-catenin. (E) and (F) Western blots (E) and co-immunoprecipitation assays (F) showing the suppressive effect of RON siRNA the interaction between HIF-1α and the RON/RONΔ160‑β-catenin complex. (G) Western blots showing levels of RON, RONΔ160, β‑catenin, and HIF-1α in MGC-803 cells overexpressing RON of RONΔ160. Scale bar = 50 μm in all panels.