Figure 9. Biological impact of endo-siRNA target gene knockdown and HSET inhibition. To confirm the biological significance of the endo-siRNA-mediated knockdown, RNA9878, GV oocytes were microinjected with a synthetic RNA9878 small RNA mimic, a non-targeting negative control, or subjected to pharmacological HSET inhibition (AZ82; 10 μM). (A) Non-targeting, RNA9878 mimic injected, and HSET inhibited oocytes were then subject to IVM to MII where (A, C) PBE and (B, D) aneuploidy rates were recorded. siRNA-mediated knockdown and HSET inhibition experiments were repeated on three biological replicates, with each replicate comprising a minimum of 20 oocytes randomly sampled from a pool of oocytes isolated from three animals. Values are shown as a mean of each replicate ± SEM. * Statistical analyses were performed using Student’s t-test, p < 0.05, ** p < 0.01. AU, arbitrary units.