Research Paper Volume 11, Issue 9 pp 2822—2835

Phloretin protects against cardiac damage and remodeling via restoring SIRT1 and anti-inflammatory effects in the streptozotocin-induced diabetic mouse model

Figure 2. Phloretin reduced hyperglycemia-induced biochemical indicators, improved histological abnormalities and cardiac hypertrophy of diabetic cardiomyopathy. (A) The serum level of AST. (B) The serum level of CK-MB. (C) The ratio of heart weight/body weight. (D) Representative histomorphometric images for the haematoxylin and eosin staining (H&E) of formalin-fixed myocardial tissues from each group (400x magnification). (E-F) Western blot analysis for the protein expression of ANP in the myocardial tissues was performed. (G) The mRNA expression of the pro-hypertrophic gene ANP in myocardial tissues was detected by RT-qPCR. More than five mice in each group were used for above analysis. *P < 0.05, **P < 0.01 v.s. T1DM group; #P < 0.05, ##P < 0.01 v.s. CTRL group.