Figure 3. Phloretin reduced hyperglycemia-induced fibrosis in the heart of diabetic mice. (A) Myocardial tissues from each group were subjected to Masson’s Trichrome staining and Sirius red staining assay as described in Methods. (B-C) Western blot analysis for the protein expression of COL-1A1 and TGF-β in the myocardial tissues, with GAPDH as a loading control. (D-F) The mRNA expression levels of COL-1A1 and TGF-β in myocardial tissues of each group were determined by real-time qPCR. More than five mice in each group were used for above analysis. *P < 0.05, **P < 0.01 v.s. T1DM group; #P < 0.05, ##P < 0.01 v.s. CTRL group.