Figure 4. Phloretin reduced hyperglycemia-induced inflammation in the heart of diabetic mice. (A) Heart tissues were subjected to IHC analysis with anti-F4/80 antibodies as described in Methods (400x magnification). (B-C) Western blot analysis for the IκB degradation in the myocardial tissues, with GAPDH as a loading control. (D) The mRNA expression levels of IL-6 in myocardial tissues of each group were determined by real-time qPCR. More than five mice in each group were used for above analysis. *P < 0.05, **P < 0.01 v.s. T1DM group; #P < 0.05, ##P < 0.01 v.s. CTRL group.