Research Paper Volume 11, Issue 9 pp 2822—2835

Phloretin protects against cardiac damage and remodeling via restoring SIRT1 and anti-inflammatory effects in the streptozotocin-induced diabetic mouse model

Figure 7. Molecular docking analysis of PHL within binding site of SIRT1 at SIRT1: PHL binding ratios of 1:1, 1:2, 1:3, and 1:4. (A) 1:1; (B) 1:2; (C) 1:3; (D) 1:4; (E) The distribution of 100 conformational scores for each ratio; (F) The detailed view of SIRT1: PHL with the binding ratio of 1:3. (G-H) Total proteins (100 μg) were extracted from the cardiac tissues in CTRL, T1DM and T1DM+PHL group. The expression level of SIRT1 in total protein was examined by western blot, with GAPDH as a loading control. Data are presented as means ± SEM. *P < 0.05 v.s. HG group; #P < 0.05 v.s. DMSO group.