Figure 6. Effects of hTERT and SV40LT transfection on the DNAm age of fibroblasts. These panels show DNAmAge391CpG (A–D) and DNAmAge353CpG (E–H) of fibroblasts transfected with the hTERT and SV40LT genes, respectively. The changes of DNAm age more than 20 PD after hTERT (A and E) or SV40LT (B and F) gene transfection are shown. The dots with the same color are the results from the same cell line. Panels (C and D) show DNAmAge391CpG/PD (C) and DNAmAge353CpG/PD (G) of each cell line transfected with the hTERT or SV40LT gene. As references, the DNAm age/PD values of non-transfected cells are shown for 3 cell lines. The starting DNAm age values are shown on the X axis. Panels (D and H) show the average and S.E. of DNAmAge391CpG/PD (D) and DNAmAge353CpG/PD (H) of non-transfected control cells (n=3), hTERT (n=5), and SV40LT (n=5). The effects of SV40LT were not consistent, and the S.E. of the average is very large.