Figure 4. The correlation between HMMR-AS1 and miR-138. (A) HMMR-AS1 expression was detected in the cytoplasm (green) and nuclear fractions (blue) of NCI-H23 and A549 cells by FISH analysis. (B, C) The luciferase reporter plasmid containing WT/Mut HMMR-AS1 was co-transfected into HEK-293T cells. (D) RNA levels in immunoprecipitates are presented as fold enrichment in Ago2 relative to IgG immunoprecipitates. (E, F) miR-138 expression in NCI-H23 and A549 cells was detected by qRT-PCR analysis. (G) Correlation between HMMR-AS1 and miR-138 expression in 18 paired LUAD tissues. *P < 0.05, **P < 0.01.