Research Paper Volume 11, Issue 10 pp 3041—3054

LncRNA HMMR-AS1 promotes proliferation and metastasis of lung adenocarcinoma by regulating MiR-138/sirt6 axis

Figure 5. Effects of miR-138 on proliferation, apoptosis and cell cycle of LUAD cells. (A) MiR-138 expression in NCI-H23 and A549 cells was detected by qRT-PCR after transfection with miR-138 mimics or inhibitor. (B) Cell proliferation was inhibited by miR-138 mimics showed by CCK8 assays. (C, D) Apoptotic rate and cell cycle arrest in A549 and NCI-H23 cells were detected by flow cytometry. (E) NCI-H23 cell viability after co-transfection was determined by CCK8 assay. (F) NCI-H23 cell proliferation was determined by colony formation assays. *P < 0.05, **P < 0.01.