Figure 5. Kinetics of primer MARylation in Mmu WCE (A) and quantification of the reaction products (B). The Mmu cell extract proteins (0.5 mg/mL) were incubated for 5, 10, or 20 min with 100 nM DNA duplexes bearing dRP, pDEG, or flap in the presence of 0.5 mM NAD+ and 5 mM spermine as described in the section ‘DNA (ADP-ribosyl)ation assay’. Lane 1 corresponds to the initial primer (control). The yield of the MARylated primer (%) was calculated as the amount of the corresponding product normalized to overall DNA content in the lane.