Research Paper Volume 11, Issue 9 pp 2852—2873

Poly(ADP-ribosyl)ation and DNA repair synthesis in the extracts of naked mole rat, mouse, and human cells

Figure 5. Kinetics of primer MARylation in Mmu WCE (A) and quantification of the reaction products (B). The Mmu cell extract proteins (0.5 mg/mL) were incubated for 5, 10, or 20 min with 100 nM DNA duplexes bearing dRP, pDEG, or flap in the presence of 0.5 mM NAD+ and 5 mM spermine as described in the section ‘DNA (ADP-ribosyl)ation assay’. Lane 1 corresponds to the initial primer (control). The yield of the MARylated primer (%) was calculated as the amount of the corresponding product normalized to overall DNA content in the lane.