Figure 3. Overexpression of miR-320a enhanced hyperglycemia induced podocytes injury in vitro. (A) Relative miR-320a expression in cultured podocyte cells exposed to normal glucose (NG, 5 mM) and high glucose (HG, 30 mM). (B) In vitro permeability of FITC-labeled BSA through podocyte monolayers. (C) Representative photomicrographs of immunofluorescence labeling with F-actin in cultured podocyte cells. Effects of miR-320a mimics on apoptosis (D) and ROS (E) were determined by Annexin V/PI flow cytometric analysis and DHE in cultured podocyte cells. Data are representative of three experiments. Data are expressed as mean ± SEM, n=3, *P<0.05 versus NG, #P<0.05 versus HG + miR-con, &P<0.05 versus HG + inhibitor-con.