Research Paper Volume 11, Issue 9 pp 2898—2910

miR-495 inhibits the growth of fibroblasts in hypertrophic scars

Figure 2. Relationship between miR-495 and FAK. (A) miRDB predicted that miR-495 specifically combined with FAK mRNA. (B) Expression of FAK in hypertrophic scar tissues and normal tissues were detected using real-time PCR. ** P< 0.01 vs. normal tissues. (C) Levels of FAK expression in hypertrophic scar fibroblasts, normal scar fibroblasts and normal fibroblasts were detected using real-time PCR. ** P< 0.01 vs. fibroblasts, ## P< 0.01 vs. scar fibroblasts. (D) Correlation between expression of miR-495 and FAK in hypertrophic scar. (E) Interaction between miR-495 and the FAK 3’-UTR was tested in luciferase reporter assays. Data are presented as the mean ± SEM. ** P< 0.01 vs. control. (F, G) Western blotting and real-time PCR showing that when miR-495 is overexpressed, FAK expression is downregulated. Data are presented as the mean ± SEM. ** P< 0.01 vs. control. (H, I) Western blotting and real-time PCR analysis showing that when miR-495 is repressed, FAK expression is upregulated. Data are presented as the mean ± SEM. ** P< 0.01 vs. control.