Research Paper Volume 11, Issue 9 pp 2898—2910

miR-495 inhibits the growth of fibroblasts in hypertrophic scars

Figure 4. FAK is crucial to the inhibitory effect of miR-495 on HSFs growth. (A) MTT assays showing the effects of si-FAK and miR-495 inhibitor on HSF proliferation. Data are presented as the mean ± SEM. ** P< 0.01 si-FAK vs. control, ## P< 0.01 si-FAK+miR-495 inhibitor vs. si-FAK. (B) AV-PI assays showing the effects of si-FAK and miR-495 inhibitor on HSF apoptosis. (C, D) Following transfection of HSFs with si-FAK or si-FAK plus miR-495 inhibitor, expression of FAK, FAKp-Tyr397, cyclin D1, bcl-2, bax and COL1A were detected using western blotting and real-time PCR. Data are presented as the mean ± SEM. ** P< 0.01 si-FAK vs. control, ## P< 0.01 si-FAK+miR-495 inhibitor vs. si-FAK.