Figure 7. Resveratrol treatment suppressed the Akt/mTOR pathway in young zebrafish retinas. (A) Representative western blot showing the levels of p-mTOR and p-Akt-T308 in adult zebrafish retinas after being treated with resveratrol for 1 and 10 days. (B) Densitometric mean and SEM of p-mTOR and p-Akt-T308 normalized to the corresponding level of the loading control protein beta-actin (*P<0.05 compared to control (CON), n=3). (C) Photographs of fluorescence immunostained retina cross-sections showing the localization and relative levels of p-mTOR in the RGC layers of adult zebrafish retinas with or without resveratrol treatment for 10 days. All photographs were taken at 40x magnification. CON, control; RES1d, resveratrol treated for 1 days; RES/RES10d, resveratrol treated for 10 days; GCL, ganglion cell layer.