Figure 1. Small molecular inhibitor IN04 binds to the predicted active pocket of hLRRK1 kinase domain. (A) Predicted structure of hLRRK1 kinase domain with an active pocket. (B) A molecular structure of the potential LRRK1 inhibitor IN04. (C) A potential small molecular weight inhibitor docks to the active pocket of hLRRK1 KD. (D) Purified 34 kD recombinant protein of hLRRK1 expressed in E. coli stained with Coomassie blue. (E) IN04 inhibits ATP binding to the LRRK1 KD, measured by an in vitro pulldown assay.