A small molecular inhibitor of LRRK1 identified by homology modeling and virtual screening suppresses osteoclast function, but not osteoclast differentiation, <i>in vitro</i>

Mingjue Si; Canjun Zeng; Helen Goodluck; Sandi Shen; Subburaman Mohan; Weirong Xing

doi:doi:10.18632/aging.101977

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Research Paper Volume 11, Issue 10 pp 3250—3261

A small molecular inhibitor of LRRK1 identified by homology modeling and virtual screening suppresses osteoclast function, but not osteoclast differentiation, in vitro

Figure 2. IN04 treatment inhibits osteoclast bone resorption function with no effect on osteoclast formation. Osteoclast precursors derived from C57BL/6J mice were seeded on bone slices (0.4 x 0.8 cm) and differentiated in the presence of DMSO or INO4 for 6–9 days. Cells were stained for TRAP (Tartrate-resistant acid phosphatase), and bone slices were stained with hematoxylin for bone resorption pits. (A) Representative images of TRAP-positive osteoclasts (upper panel) and resorption pits (lower panel) on bone slices. (B) Quantitative data of osteoclast numbers on bone slices. (C) Quantitative data of osteoclast size on bone slices. [29]. Quantitative data of pit formation on bone slices. The results are presented as percentage of pit area relative to the bone slice area. Data are presented as mean ± SEM. P < 0.05 or P < 0.01 indicates statistical significance (N=4).