Figure 2. IN04 treatment inhibits osteoclast bone resorption function with no effect on osteoclast formation. Osteoclast precursors derived from C57BL/6J mice were seeded on bone slices (0.4 x 0.8 cm) and differentiated in the presence of DMSO or INO4 for 6–9 days. Cells were stained for TRAP (Tartrate-resistant acid phosphatase), and bone slices were stained with hematoxylin for bone resorption pits. (A) Representative images of TRAP-positive osteoclasts (upper panel) and resorption pits (lower panel) on bone slices. (B) Quantitative data of osteoclast numbers on bone slices. (C) Quantitative data of osteoclast size on bone slices. . Quantitative data of pit formation on bone slices. The results are presented as percentage of pit area relative to the bone slice area. Data are presented as mean ± SEM. P < 0.05 or P < 0.01 indicates statistical significance (N=4).