Figure 6. IN04 treatment does not influence osteoclast differentiation and coupling factor expression. Osteoclast precursors derived from C57BL/6J mice were cultured in 6-well plates and differentiated in the presence of DMSO or INO4 for 6–9 days followed by RNA extraction and real-time RT-PCR. Expression levels of endogenous Lrrk1 and osteoclast differentiation markers NFATc1, Acp5, and Cathepsin K, respectively (N=6). Expression levels of osteoclast coupling factors BMP6 (bone morphometric protein 6), CTHRC1 (collagen triple helix repeat containing 1), and Wnt10b (wingless-type MMTV integration site family, member 10B), respectively (N=6). Data are presented as mean ± SEM. P < 0.05 or P < 0.01 indicates statistical significance.