Research Paper Volume 11, Issue 10 pp 3250—3261

A small molecular inhibitor of LRRK1 identified by homology modeling and virtual screening suppresses osteoclast function, but not osteoclast differentiation, in vitro

Figure 6. IN04 treatment does not influence osteoclast differentiation and coupling factor expression. Osteoclast precursors derived from C57BL/6J mice were cultured in 6-well plates and differentiated in the presence of DMSO or INO4 for 6–9 days followed by RNA extraction and real-time RT-PCR. Expression levels of endogenous Lrrk1 and osteoclast differentiation markers NFATc1, Acp5, and Cathepsin K, respectively (N=6). Expression levels of osteoclast coupling factors BMP6 (bone morphometric protein 6), CTHRC1 (collagen triple helix repeat containing 1), and Wnt10b (wingless-type MMTV integration site family, member 10B), respectively (N=6). Data are presented as mean ± SEM. P < 0.05 or P < 0.01 indicates statistical significance.