Figure 7. IN04 treatment has no effect on nodule formation in vitro. Bone marrow stromal cells were treated with a mineralization medium containing 10 mM β-glycerophosphate, 50 μg/ml ascorbic acid, and 10% FBS in the presence of DMSO or INO4 for 24 days and stained with alizarin red. (A) Images of nodule formation. (B) Quantitative mineralization area measured by the OsteoMeasure system. Data are presented as mean ± SEM, N=3.